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Calcein Protocol

Calcein, Sigma C-0875.
1% solution of calcein +2% sodium bicarbonate in saline
Stir 4 hr, filter. Inject: 10 mg/kg

For 50 mL:
  • 0.5 g Calcein
  • 1 g sodium bicarbonate
  • 50 mL Saline
10 mg/mL soln:
inject 10 mg/kg
= 1 mLkg
= 1 uL/g
so...20 g mouse, inject 20 µL
 
thus dilute stock 1:10 before injections, giving 200 µL instead