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Oil Red-O Staining of Adipocytes

  1. Prepare Oil Red-O: Stock is 0.5% in isopropanol. To make working solution, add 60 mL stock to 40 mL H2O (or anything with that ratio), remove precipitate with Whatman 40 filter 
  2. Wash plate 1 time with 1x PBS 
  3. Fix cells with 3.7% Formaldehyde for 2 minutes on rocker
  4. Wash 1 time with H2
  5. Add Oil Red-O Working Solution (3 mL/10 cm plate; 1 mL/6 cm plate) and incubate for 1 hr at room temperature on rocker
  6. Aspirate Oil Red-O and wash with H2O; store in H2O and Sodium Azide