Protocols/Methods

1x Scalable Yeast Transformation Protocol

3T3-L1 Differentiation Protocol

Adipose tissue lysis for western analysis

Agarose Gel Electrophoresis

Assay for Free Cytosolic b-catenin

b-oxidation assay

Bromodeoxyuridine (BrdU) Incorporation

Calcein Protocol

C/EBPα Blot

Chromatin Immunoprecipation (ChIP) assay for 3T3-L1 preadipocytes

Dephosphorylation of DNA ends with Calf Intestinal Alkaline Phosphatase (CIP)

Digestion for Glycogen determination in liver/muscle

DNA ligation

DNA Sequencing Protocol

Freezing Down Cells

EMSC (Ears Mesenchymal Stem Cells) Isolation and Culture

General Cell and Tissue Lysis

Gitschier Protocol for Genomic DNA isolation from mice

Growing Up Plasmids

High Titer Retroviral Infections Using Virus with VSVG

Histology of Adipose Tissue

Immunoblot Protocol

Immunohistochemistry of Paraffin Sections

Immunoprecipitation Protocol

Insulin Stimulated Glucose Uptake Assay

Ken's Lysis Buffer

Library-scale transformation of yeast

Oil Red-O Staining of Adipocytes

RNA extraction using RNeasy kit

Skin Sample Preparation

Tail Digest

Western Blot