Agarose Gel Electrophoresis
*(KA Longo, 1/01)
Assemble gel plate and comb in the gel box, and pour enough molten agarose into the apparatus until it is about 8 mm deep. Allow to cool and harden for 20-30 minutes.
Reposition the gel plate (with gel intact) inthe gel box to facilitate electrophoresis, and add enough 1 x TAE to immerse the gel.
Add the proper volume of 6 x Loading buffer to your DNA samples, mix thoroughly, and spin briefly.
Load your samples in the gel wells, including a molecular weight standard.
Run your gel at constant voltage, 120-160 volts, for 30-60 minutes.
Power down and remove the gel.
DNA in the gel can now be visualized using a UV light source. A picture should betaken to document your experiment.
Dump the waste TAE buffer into the sink.
Rinse the gel box and plate with tap and distilled water. When the aparatus is dry, return it to the drawer.
Dispose of your gel in the ethidium bromide waste bucket.