Calcein Protocol

Calcein, Sigma C-0875.

1% solution of calcein +2% sodium bicarbonate in saline

Stir 4 hr, filter. Inject: 10 mg/kg

For 50 mL:

• • 0.5 g Calcein

  • 1 g sodium bicarbonate

  • 50 mL Saline

10 mg/mL soln:

inject 10 mg/kg

= 1 mLkg

= 1 uL/g

so...20 g mouse, inject 20 µL

thus dilute stock 1:10 before injections, giving 200 µL instead